RNA

Part:BBa_K2923016

Designed by: Kateryna LEN   Group: iGEM19_Strasbourg   (2019-10-13)


Two PP7 stem-loops

Our bacterial three-hybrid (BH3) construct (inspired by Berry and Hochschild, 2018) is composed of two fusion proteins with MS2 and PP7 coat protein and RNA. This present part is composed of two PP7 (Pseudomonas phage PP7) stem-loops. PP7 stem loops are able to be specifically recognized by PP7 coat proteins for linked all the parts of our construct together. This part permits to create a new RNA sequence bordered by MS2 and PP7 stem-loops.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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